诱导K562细胞分化对EDAG基因表达的影响
基因表达,1材料与方法,2结果,3结论,参考文献：
摘 要：目的 研究EDAG在红系肿瘤分化中的表达及意义。 方法 用PCR的方法从人的血液基因组中分离出EDAG基因的调控区2 200 bp片段，将其克隆到pGL3basic的含有荧光素酶报告基因的质粒中，转染K562细胞，用Hemin和PMA诱导细胞分化；通过不同时间检测荧光素酶报道基因的方法，观察用两种不同方法处理细胞后对EDAG基因表达的影响。 结果 用Hemin和PMA分别诱导K562 72 h和48 h后，EDAG基因表达下调。 结论 用Hemin和PMA诱导K562细胞分化后，EDAG基因表达下调可能与细胞分化有关。关 键 词： EDAG；基因表达；诱导分化
K562 cells affect on EDAG genic expression by inductor
FANG Fang1, CHENG Zhonghang2, ZHANG Zhenhao1, XU Wangxiang 3, MA Aixin1
(1.Department of Etiology, 2.Department of StateOwned Assets, Jilin Medical College, Jilin City, Jilin Province, 132011; 3.Institute of Radiation Medicine, Academy of Military Medicine Sciences, Beijing, 100850, China)
Abstract： Objective To study EDAG gene expression in differentiation of k562 cells. Methods 2 200 bp fragment of the EDAG promoter region was isolated from human blood genome. PCR products were subcloned into the luciferase reporter vector pGL3basic and transfect with K562 cells. K562 cells were induced by PMA and Hemin, the expression of EDAG gene in the resulted cells was determinded by luciferase assay. Results The expression of EDAG gene in K562 cells was downregulated by Hemin and PMAinducing for 72 and 48 hours respectively. Conclusion Expressional downregulation of EDAG gene is related to be cell differentiation by PMA and Hemin inducing. ......
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