Effects of nisoldipine on the myocardial remodeling of left ventricle in renal hypertensive rats

    CHEN Hui, ZHANG Ling, ZHU Dan, ZHOU Yiª²Wen, ZOU Lin,TU Zhiª²Guang

    Faculty of Laboratory Medicine, Chongqing University of Medical Sciences, Chongqing 400016, China

    ¡¾Abstract¡¿ AIM: To study the effects of nisoldipine on the myocardial remodeling of left ventricle in renal hypertensive rats (RHR). METHODS: RHR model was established with nisoldipine (10 mg/kg) ig. 12 h for 30 d and the index of heart was compared between groups. A piece of tissue from the middle of left ventricle was harvested and the paraffin sections and ultrathin sections were prepared routinely. The changes of microstructural and ultrastructural were observed respectively. Picrosirus red staining was performed and the changes of the collagen contents and type ¢ñ to type ¢ó ratio were determined. RESULTS: Nisoldipine decreased the ratios of heart mass/body mass and left ventricle mass/body mass slightly (by 5.4 % and 7.7 % respectively, P>0.05), stopped the myocardium from necrosis and inflammatory infiltration, and alleviated myocardial hypertrophy. Under transmission electron microscope, the proliferation and swelling of mitochondria were reduced obviously, the Z lines were partially broken, but the field with notable fibrosis was not found. Nisoldipine significantly reduced the collagen volume fraction and the integrity optical density (by 63.0 % and 62.8 % respectively, P<0.05) and kept the ratios of collagen type ¢ñ to collagen type ¢ó from inverting. CONCLUSION: Nisoldipine plays a protective role in the left ventricle remodeling of hypertension, especially in attenuating the hyperplasia of collagen. Nisoldipine reduces the blood pressure and improves the myocardial structure simultaneously. ......