Effects of transfecting bclª²2 gene into cardiomyocytes on apoptosis of the cells during ischemia/reperfusion injury

    LI Junª²Xia, JIA Guoª²Liang, JIN Ming, MA Yueª²Yun£¬ SU Mingª²Quan

    1Department of Cardiovasology, Bethune International Peace Hospital, Shijiazhuang 050082, China, 2Department of Cardiovasology, Xijing Hospital, 3Department of Biochemistry and Molecular Biology, School of Basic Medicine, 4Center of Clinical Molecular Biology of Laboratory, Xijing Hospital, Fourth Military Medical University, Xi¡¯an 710033, China

    ¡¾Abstract¡¿ AIM: To observe the effects of transfecting antiapoptosis gene into cardiomyocytes on the apoptosis of the cells during ischemia/reperfusion injury. METHODS: A cell culture model of neonatal rat cardiac myocytes was used and the retrovirus eukaryotic expression vector pDORª²SB containing human bclª²2 gene was extracted and identified by agarose gel electrophoresis after being cut with restricted endonuclease EcoR¢ñ. The pDORª²SB was introduced into cardiomyocyte cells by liposomeª²mediated gene transfection method. Three groups were adopted: control group, simulated ischemia/reperfusion group and gene transfecting group, and apoptosis was determined by transmission electron microscopy (TEM). RESULTS: Apoptosis was not found in the control group. After 120 min of simulated ischemia followed by 240 min of reperfusion, typical apoptotic cells were seen by TEM, while in bclª²2 gene transfecting group, apoptosis decreased. CONCLUSION: Cardiomyocyte apoptosis is involved in the ischemia/reperfusion injury and transfecting bclª²2 gene into cardiacmyocytes can significantly inhibit cardiomyocyte apoptosis during myocardial ischemia/reperfusion injury. ......