Effect of simulated pushª²pull maneuver to different degrees on expression of bFGF in hippocampus neurons of rats

    GUO Suª²Hong, WANG Xiaoª²Yu, LI Zhiª²Xin, ZHU Xinª²Wei, LI Yunª²Yi, X¨¹ Bin

    Department of Laboratory Techniques, Jilin Military Medical College, Fourth Military Medical University, Jilin 132013, China

    ¡¾Abstract¡¿ AIM: To establish a stimulated pushª²pull maneuver animal model through animal centrifugal animal and to explore the effect of pushª²pull effect on bFGF expression of hippocampus neurons in rats. METHODS: Forty healthy male Wistar rats were randomly divided into 10 groups (the control group, 0.5 h, 6 h, 24 h groups after 3 degrees of exposure) and there were 4 rats in each group. They were perfused and fixed and then the brain hemispheric tissues were taken and sectioned for bFGF determined of neurons in different hippocampus areas in rats through immunoª²histological chemical staining and image analysis techniques. RESULTS: In control group and 30 min groups after ¡À2Gz exposure, bFGF had weak positive expression. In the 30 min experimental groups after ¡À6Gz, ¡À8Gz exposure,bFGF expression was increasing while bFGF expression of 6 h groups was clearly increased, and 24 h later, the expression was decreased, but still higher than that in the normal control group. There was a significant difference in comparison with the control group (P<0.05). CONCLUSION: Middle and high intensity pushª²pull maneuver may cause an increase of the bFGF expression of hippocampus neurons. ......