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编号:10871452
HSVtk/GCV自杀基因系统对骨肉瘤细胞体外杀伤效应
http://www.100md.com 《第四军医大学学报》 2004年第3期
骨肉瘤,,骨肉瘤;HSVtk基因;基因治疗,0引言,1材料和方法,2结果,3讨论,【参考文献】
     Killing effect of GCV combined with suicide gene HSVtk on human osteosarcoma cells in vitro

    HU TieShan, LI GuiTao

    Department of Orthopaedics, 177 Hospital, Guangdong Province, Guangzhou 510317, China

    【Abstract】 AIM: To investigate the killing effect of GCV combined with suicide gene HSVtk on SAOS2 cells in vitro. METHODS: Retroviral expressing vector pL(tk)SN was generated by recombinant DNA technique. SAOS2 was infected by the recombinant retrovirus expressing tk gene. The positive clones were obtained by G418 selection and termed as SAOS/tk. The integration and expression of tk gene in SAOS2 cells were identified by RTPCR and Southern blot. The growth state and prodrugs’ killing effect of tk gene modified cells were investigated with the expression of tk gene and antitumor effect on SAOS2 cells. RESULTS: RTPCR and Southern blot analysis confirmed the integration and expression of tk gene in retrovirus transduced SAOS2 cells. There was no significant difference in cell proliferation between the SAOS/tk and SAOS2. SAOS/tk showed high sensitivity to GCV and bystander effect in vitro. CONCLUSION: The osteosarcoma cells SAOS2 are sensitive to HSVtk/GCV system and have significant bystander effect, which might have therapeutic potentials for osteosarcoma. ......

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