Adsorptive saturation time of different filter membranes on doing hemofiltration

    JIANG Hongª²Li, XUE Wuª²Jun, YIN Aiª²Ping, LI Daª²Qing, FENG Xueª²Liang, KONG Shu, JU Fengª²Chi

    1Department of Hemodialysis Center, First Hospital, Xi¡¯an Jiaotong University, Xi¡¯an 710061, 2Department of Biomedicine Engineering, Xi¡¯an Jiaotong University, Xi¡¯an 710049, China

    ¡¾Abstract¡¿ AIM: To observe adsorptive saturation time of different filter membranes in vitro and provide theoretical foundation for the time of changing filter when hemofiltration was carried out. METHODS: 2000 mL cattle blood was collected into one blood bag. Then the blood was stimulated for 12 h at 37¡æ with 1 mg/L endotoxin to induce inflammatory mediators. Cattle blood was divided into 3 groups (AN69£¬PMMA£¬PS group) and hemofiltration was performed in vitro. AN69£¬PMMA£¬PS filter membrane resistance levels were online measured at different time point using Data Bridge. Plasma TNF¦Á concentration was measured by ELISA. RESULTS: Resistance level of AN69, PMMA, PS group presented changing tendency with time going (P<0.01). The highest resistance level was at 120, 90, 90 min time point in AN69, PMMA, PS group respectively. Resistance level of filter membrane was difference in different group (P<0.05). Plasma TNF¦Á concentration of AN69, PMMA, PS group also presented changing tendency with time going (P<0.01). The lowest level of plasma TNF¦Á concentration was at 120, 90, 90 min time point respectively. Plasma TNF¦Á concentration was difference in different group (P<0.05). TNF¦Á concentration decreasing was significantly correlated with resistance level increasing. r value was ¨C0.778, -0.816, -0.915, -0.960, -0.966, -0.958 respectively (P<0.01).CONCLUSION: Membrane resistance level online measured by Data Bridge could instantly reflect the degree of membrane adsorption. Adsorptive saturation time of different membranes was different. Blood flow were 200 mL/min and filtrate flow were 2000 mL/h, adsorptive saturation time of AN69, PMMA, PS membrane were at 120, 90, 90 min, respectively. When adsorption amount of AN69 membrane were the biggest. ......