黏液表皮样癌细胞_

重组人肿瘤坏死因子α诱导黏液表皮样癌细胞凋亡

http://www.100md.com 《第四军医大学学报》 2004年第1期

黏液表皮样癌细胞,,重组人肿瘤坏死因子α；凋亡；黏液表皮样癌细胞,0引言,1材料和方法,2结果,3讨论,【参考文献】

     Apoptosis of mucoepidermoid carcinoma cells induced by recombinant human tumor necrosis factorα

    LIU DaQing, SITU ZhenQiang, ZHOU ShuXia, CAO YunXin, XU YanMing, YU CuiJuan, WANG ChengJi, YANG AnGang

    1Department of Oral and Maxillofacial Surgery, Stomatological College, 2Department of Biochemistry and Molecular Biology, 3Department of Immunology, School of Basic Medicine, Fourth Military Medical University, Xi’an 710033, China

    【Abstract】 AIM: To explore the apoptosis mechanism of mucoepidermoid carcinoma cells (MEC1) induced by recombinant human tumor necrosis factorα (rhTNFα). METHODS: By cell counting, the qualitative analysis of DNA fragmentation, the assays of the morphological changes and the cell cycle change, we evaluated the growth condition and apoptosis induction of the MEC1 cells treated with rhTNFα. RESULTS: Treated with rhTNFα, the living MEC1 cell numbers decreased and massive of apoptotic cells occurred, DNA fragmented into nucleosemal oligomers,agarose gel eletrophoresis of fragmented DNA showed a ladderlike pattern; the cell cycle changed and electron microscopic observation showed chromatin aggregating beneath the nuclear envelop, nuclear contraction. CONCLUSION: rhTNFα exerts its cytotoxic effects on the MEC1 through inducing the cells apoptosis. ......

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