脐血_____脐血；多潜能干细胞；单个核细胞；细胞分化

人脐血PSC3445多潜能干细胞体外分离培养与鉴定

http://www.100md.com 《第四军医大学学报》 2004年第11期

脐血,,脐血；多潜能干细胞；单个核细胞；细胞分化,0引言,1材料和方法,2结果,3讨论,【参考文献】

     Isolation, culture, and propagation of human umbilical cord blood PSC3445 pluripotent stem cells

    ZHAO ShuPing, SHEN Qian1, YU LiHua, ZHANG Jun, LI WenJie, XU YuLian, YE Liang

    1Experimental and Diagnostic Department, Changhai Hospital, Shanghai 200433, China, 2Central Laboratory, 88th Hospital of Chinese PLA, Tai’an 271000, China

    【Abstract】 AIM: To culture, identify, characterize and propagate human umbilical cord blood PSC3445 pluripotent stem cells. METHODS: Fresh human umbilical cord blood mononuclear cells were obtained after FicollHypaque fractionation and two to three washes with PBS and the cells were incubated at Potentculture for 10-14 d at 37℃ (50 mL/L CO2). The cells were determined by FACScan flow analysis with CD14PE, CD11bPE, CD45FITC, CD34PE, CD3FITC, CD105PE, CD166PE, CD29PE, CD90FITC, CD33PE, CD41PE, CD44FITC, CD7FITC, CD61FITC and HLADRPE, and stained for PAS, AP, Alk.P, and POX. RERULTS: PSC3445 cells expressed CD14, CD45, CD29, HLADR and CD11b, but did not express CD34, CD105, CD166, CD90 and CD3. The cells displayed PAS+, AP+, Alk.P-, ORO- and POX- by special chemical staining and could be induced to differentiate into T cells, macrophages, epithelial cells, neuronallike cells by appropriate cell growth factor. CONCLUSION: The cells, fibroblastslike cells, are novel pluripotent stem cells in human umbilical cord blood. ......

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