内皮生长因子_____内皮生长因子；绿色荧光蛋白；心肌

pIRES2EGFPhVEGF165真核表达载体的构建及在心肌细胞内的表达

http://www.100md.com 《第四军医大学学报》 2004年第12期

内皮生长因子,,内皮生长因子；绿色荧光蛋白；心肌,细胞学,0引言,1材料和方法,2结果,3讨论,【参考文献】

     Construction of pIRES2EGFPhVEGF165 eukaryotic expression plasmid and its expression in cardiac myocytes in vitro and vivo

    ZHAO ZhiJing, Lü AnLin, JIA GuoLiang, CHEN GuangSheng, YE Jing

    1Department of Cardiovasology, Xijing Hospital, 2Department of Pathology, School of Basic Medicine, Fourth Military Medical University, Xian 710033, China

    【Abstract】 AIM: To construct a eukaryotic expression plasmid pIRES2EGFPhVEGF165 and to transfect it into cardiac myocytes in vitro and vivo. METHODS: The hVEGF165 DNA fragment was inserted into a green fluorescent protein fusion vector, which was then transfected into cardiac myocytes in vitro and vivo. RESULTS: A recombinant eukaryotic expression plasmid pIRES2EGFPhVEGF165 was constructed and was successfully transfected into cardiac myocytes. The expression of VEGF165 was confirmed by fluoroscopy and immunohistochemical staining. CONCLUSION: VEGF can be expressed stably in cardiac myocytes, which might be useful for gene therapy of myocardial ischemia. ......

百拇医药网 http://www.100md.com/html/DirDu/2006/02/19/87/14/96.htm

您现在查看是摘要页，全文长 9043 字符。