脊髓损伤;__(期刊论文)

神经营养素3对大鼠急性脊髓损伤后血中一氧化氮合成酶的影响

http://www.100md.com 《中国矫形外科杂志》 2005年第22期

脊髓损伤;,,神经营养因子3；脊髓损伤;一氧化氮合成酶,1材料与方法,2结果,3讨论,参考文献：

     摘要：[目的]探讨NT3(神经营养素3)对脊髓损伤保护作用的机制。[方法]105只SD大鼠随机分为3组：对照组(生理盐水组)，实验组(NT3组)和假手术组，每组35只，各分为7个时相点，每个时相点5只大鼠。采用Allen’s法以30gcm力致伤大鼠T8脊髓，经蛛网膜下腔导管分别于术后0、4、8、12、24h、3、7d各注入NT3溶液200ng与生理盐水组和假手术组对照，于术后4、8、12、24h、3、7、14d自右心室取血用比色法测定血清中的一氧化氮合成酶活性。[结果]大鼠脊髓损伤后一氧化氮合成酶(NOS)升高，术后12h达高峰，实验组NOS水平及升幅明显低于对照组。[结论]NT3能有效抑制NOS在脊髓损伤后的活性，从而减少了NO的生成，这可能是促进大鼠脊髓损伤后运动功能恢复的作用机制之一。

    关键词：神经营养因子3；脊髓损伤;一氧化氮合成酶

    Effect of neurotrophin3 on blood NOS after actual spinal cord injury of rats

    GUO Shuzhang,REN Xianjun,JIANG Tao,et al.

    Department of Orthopaedics,Xinqiao Hospital of Third Military Medical University, Chongqing 400037

    Abstract：[Objective]To investigate the protective mechanism of neurotrophin3(NT3) in spinal cord injury(SCI).[Method]One hundred and five SD rats were randomly divided into 3 groups:control group(saline group),experimental group(NT3 group) and sham group. SCI in SD rats was created with Allen's method by a 30gcm impacting on the posterior T8 spinal cord. NT3 was grafted into the subarachnoid space of the rats in treatment group immediately and 4,8,12, 24h and 3,7d after spinal cord injury. The nitricoxidesynthase (NOS) activity in rats blood were assayed with colorimetric methods in 4,8,12,24h and 3,7,14d.[Result]Abnormal activity expression of NOS was detected in serum of injured rats, and reached peak at 12 hours after the injury.The level of NOS activity in NT3 group was significantly lower as compared with that in control saline group.[Conclusion]NT3 can protect spinal cord from injury in vivo,and inhibit abnormal expression of nitricoxidesynthase(NOS),thus the neurotoxicity of excessive nitricoxide(NO) is abated. ......

百拇医药网 http://www.100md.com/html/DirDu/2006/02/19/87/16/40.htm

您现在查看是摘要页，全文长 8589 字符。