重组腺病毒介导的HBV核心蛋白和hEDN融合蛋白基因在小鼠肝脏的表达
肝炎病毒,,肝炎病毒,乙型;重组融合蛋白质类;腺病毒科;重组,遗传;免疫组织化学,0引言,1材料和方法,2结果,3讨论,【参
Expression of fusion protein gene of HBV core protein and human eosinophil derived neurotoxin mediated by recombinant adenovirus in mouse liverZHAO Ya, LIU Jun, GONG WeiDong, LI YingHui, DING Jin, XUE CaiFang
Department of Etiology, School of Basic Medicine, Fourth Military Medical University, Xian 710033, China
【Abstract】 AIM: To express the recombinant adenovirus vector AdHBchEDN and the control vector AdhEDN in mouse liver respectively. METHODS: Mice were injected with high titer of AdHBchEDN and AdhEDN via tail vein and the expressions of the two recombinant adenovirus vectors in liver were studied by immunohistochemistry. RESULTS: Both recombinant adenovirus vectors were expressed successfully in mouse liver. CONCLUSION: Expression of the recombinant adenovirus vector AdHBchEDN lays the foundation for its antiHBV function study in HBV animal model.
【Keywords】 hepatitis B virus; recombinant fusion proteins; adenoviridae; recombination, genetic; immunohistochemistry
【摘要】 目的: 使HBchEDN基因重组腺病毒载体(AdHBchEDN)及其对照载体AdhEDN在小鼠肝脏获得表达. 方法: 将体外扩增获得的高滴度AdHBchEDN和AdhEDN经尾静脉注射感染小鼠 ......
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