当前位置: 首页 > 期刊 > 《第四军医大学学报》 > 2004年第12期
编号:10871986
重组腺病毒介导的HBV核心蛋白和hEDN融合蛋白基因在小鼠肝脏的表达
http://www.100md.com 《第四军医大学学报》 2004年第12期
肝炎病毒,,肝炎病毒,乙型;重组融合蛋白质类;腺病毒科;重组,遗传;免疫组织化学,0引言,1材料和方法,2结果,3讨论,【参
     Expression of fusion protein gene of HBV core protein and human eosinophil derived neurotoxin mediated by recombinant adenovirus in mouse liver

    ZHAO Ya, LIU Jun, GONG WeiDong, LI YingHui, DING Jin, XUE CaiFang

    Department of Etiology, School of Basic Medicine, Fourth Military Medical University, Xian 710033, China

    【Abstract】 AIM: To express the recombinant adenovirus vector AdHBchEDN and the control vector AdhEDN in mouse liver respectively. METHODS: Mice were injected with high titer of AdHBchEDN and AdhEDN via tail vein and the expressions of the two recombinant adenovirus vectors in liver were studied by immunohistochemistry. RESULTS: Both recombinant adenovirus vectors were expressed successfully in mouse liver. CONCLUSION: Expression of the recombinant adenovirus vector AdHBchEDN lays the foundation for its antiHBV function study in HBV animal model.

    【Keywords】 hepatitis B virus; recombinant fusion proteins; adenoviridae; recombination, genetic; immunohistochemistry

    【摘要】 目的: 使HBchEDN基因重组腺病毒载体(AdHBchEDN)及其对照载体AdhEDN在小鼠肝脏获得表达. 方法: 将体外扩增获得的高滴度AdHBchEDN和AdhEDN经尾静脉注射感染小鼠 ......

您现在查看是摘要页,全文长 11104 字符