Antiª²tumor effects of B lymphocytes on immunoreconstituted SCID mice

    JIANG Xun£¬ZHANG Zhiª²Pei, ZENG Yaoª²Ying, WANG Wenª²Liang, HUANG Liª²Jun, SHI Xingª²Yu

    1Key Laboratory of Tissue Transplantation and Immunology, Ministry of Education, Jinan University, Guangzhou 510632, China, 2Department of Pediatrics, Tangdu Hospital, Fourth Military Medical University, Xi¡¯an 710038, China, 3Department of Pathology, School of Basic Medicine, Fourth Military Medical University, Xi¡¯an 710033, China

    ¡¾Abstracts¡¿ AIM: To investigate the characteristics of establishing hepatocarcinoma models in CBA/N, nude, SCID mice and reconstituted SCID mice and to preliminarily investigate the correlation of carcinoma growth with B lymphocytes in vivo. METHODS: The human hepatocarcinoma cells cultured in vitro were inoculated into B lymphocyte deficient CBA/N mice, T lymphocyte deficient nude mice, T and B lymphocytes combined deficient SCID mice, immunoreconstituted SCID mice with BALB/c mice peripheral blood lymphocytes (Bª²PBLª²SCID)£¬and immunoreconstituted SCID mice with CBA/N mice peripheral blood lymphocytes (Cª²PBLª²SCID) and their growths were observed in the mice. The immunoglobulin Ig concentration of sera in BALB/c and Bª²PBLª²SCID mice was measured and the serum coagulation of mice with hepatocarcinnoma cell (target cells) was tested. RESULTS: There were no carcinoma growths in CBA/N and Bª²PBLª²SCID mice and their Ig immunogloblin at the level of mg/mL could be observed, but there were 100% carcinoma growths in SICD, nude and Cª²PBLª²SCID mice. Response to the carcinoma antigen in sera was observed in Bª²PBLª²SCID mice and BALB/c mice, and the response was stronger in the former than the latter. CONCLUSION: SCID mice are the most ideal experimental animals to be used for establishing the hepatocarcinoma animal model and studying tumor immunity in the various kinds of mice. B lymphocytes and their antibodies play an important antiª²tumor role. ......