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编号:10871977
汉滩病毒76118株G2S0.7嵌合基因重组腺病毒的构建及表达产物鉴定
http://www.100md.com 《第四军医大学学报》 2004年第12期
汉坦病毒,,汉坦病毒;基因表达;腺病毒科;重组,遗传,0引言,1材料和方法,2结果,3讨论,【参考文献】
     Construction and identification of recombinant adenovirus containing chimeric gene G2S0.7 of Hantaan virus

    ZHANG FangLin, LIU Yong, YU Lan, HU Gang, WU XingAn, SHI MengYuan, BAI WenTao, WANG HaiTao, XU ZhiKai

    Department of Microbiology, School of Basic Medicine, Fourth Military Medical University, Xian 710033, China

    【Abstract】 AIM: To express the chimeric gene containing M segment and 0.7 kb fragment of S segment of Hantaan virus in adenovirus expression system. METHODS: The recombinant adenovirus transfer vector pShuttleG2S0.7 was constructed and the chimeric gene was inserted into adenovirus DNA. After transfecting HEK293 cells, the recombinant adenovirus was obtained and the titer of it was determined and the expressed product was detected by ELISA. RESULTS: The recombinant adenovirus containing the chimeric gene G2S0.7 was constructed successfully and the titer of it was about 10131015pfu/L. HEK293 cells were then infected by the recombinant adenovirus and fusion protein could be expressed in the infected cells. The expressed protein could be recognized by the Hantaan virus NPspecific mAb and glycoprotein G2specific mAb. CONCLUSION: The successful expression of G2S0.7 with biological activity in adenovirus expression system lays the basis for further research on its immunological characteristics. ......

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