结肠直肠肿瘤__(期刊论文)

大肠癌耐药LoVo/Adr细胞PKC亚型与MDR的关系

http://www.100md.com 《第四军医大学学报》 2004年第3期

结肠直肠肿瘤,,蛋白激酶C；抗药性，多药；结肠直肠肿瘤,0引言,1材料和方法,2结果,3讨论,【参考文献】

     Relationship between protein kinase C isoforms and MDR in colorectal cancer multidrug resistant LoVo/Adr cells

    MA Qiang, ZHANG ZhenShu, SUN AiMin, WANG QunYing

    Department of Gastroenterology, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China

    【Abstract】 AIM: To investigate the relationship between PKC isoforms and MDR in colorectal cancer multidrug resistant LoVo/Adr cells. METHODS: Western blot was used for assessment of PKCα, PKCβ, PKCγ and PKCε expression on membrane and in cytoplasma of LoVo/Adr cells. The effect of PKC on uptake of adriamycin in LoVo/Adr cells was determined by flow cytometry. RESULTS: PKCα was mainly distributed on membrane of LoVo/Adr cells and its amount was significantly higher in LoVo/Adr cells than in LoVo ones. PKCα was transformed to membrane from cytoplasma after 30 min treatment with PMA and PKCα expression was hardly determined on membrane and in cytoplasma after 24 h treatment with PMA. The uptake of adriamycin decreased significantly from (2.04±0.70) to (1.74±0.56) after 30 min treatment with PMA (P<0.01), but increased significantly to (2.21±1.28) (P<0.01) after 24 h treatment with PMA. CONCLUSION: PKCα might be involved in MDR. ......

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