Mechanical injury model of cortical neuron of rat in vitro

    HUANG Weiª²Dong, FEI Zhou, ZHANG Xiang, WANG Xiª²Ling

    PLA Institute of Neurosurgery , Xijing Hospital, Fourth Military Medical University, Xi¬ðan 710033, China

    ¡¾Abstract¡¿ AIM: To establish a novel mechanically injured model of cortical neuron of rats in vitro. METHODS: The SD fetus (16 ª² 17 d) cortical neurons were divided into injury and control groups after 7 days in vitro cultivation. Mechanical injury was caused by plastic tip on the cortical neuronal culture in the injured group, which was subdivided into three groups according to the degree of insults, while the control group underwent all the same managements except for the mechanical injury. Cellular viability and the level of LDH were detected at different times after injury (10, 30 min, 1, 3, 6, 12 and 24 h). RESULTS: Cellular viability of injured groups was obviously lower than that of control group, which was in line with the severity of injury. The lowest level was recorded at 24 h after injury. The concentration of LDH had increased since 30 min after injury. CONCLUSION: Tipª²caused mechanical injury is easy to perform, which can simulate the pathological mechanism of traumatic brain injury, cause cortical neuronal damage at different levels and is convenient for the study of morphological changes of mechanically injured neurons and the cells around them. ......