Isolation and identification of a novel peptide binding to human transferrin receptor from phageª²display library

    QIN Xin, ZHAO Ning, ZHANG Yingª²Qi, YAN Zhen

    Biotechnology Center, Administration of Scientific Research, Fourth Military Medical University, Xi¬ðan 710033, China

    ¡¾Abstract¡¿ AIM: To select the peptide binding to human transferring receptor (hTfR) from phageª²display 12 peptides library. METHODS: Using the hTfR as the target protein, peptides that could bind with high affinity to hTfR were selected from the phageª²display 12 peptides library by biopaning. Then, phage ELISA, flow cytometry (FCM) and immonohistochemistry were employed to detect whether the selected phage could bind to hTfR and the cells possessing high expression of hTfR. RESULTS: A phage with peptide SPRPRHTLRLSL was selected. Phage ELISA, FCM and immonohistochemistry indicated that this phage could bind to hTfR and the cells possessing high expression of hTfR. CONCLUSION: A novel peptide was identified, which can bind to hTfR and probably can be used in target drug delivery system. ......