人胃癌细胞株抗脱落凋亡特性的分析
胃肿瘤,,胃肿瘤；肿瘤细胞，培养的；脱落凋亡,0引言,1材料和方法,2结果,3讨论,【参考文献】
(第四军医大学基础部：生物化学与分子生物学教研室，病理生理学教研室，陕西 西安 710033，美国波士顿大学医学院肺研究中心)Characteristics of anoikis resistance of human gastric cancer cell lines
LI Ying, HAN Jiong,WANG LiFeng, LIN ShuXin, YAO LiBo, YU Qiang, LIU XinPing*
Department of Biochemistry & Molecular Biology, Department of Pathophysiology, Fourth Military Medical University, Xian 710033, China, Pulmonary Center, Medical College, Boston University, U.S.A
【Abstract】 AIM: To analyze the characteristics of the anoikis resistance of human gastric cancer cell lines(HGC27, BGC823, MGC803 and SGC7901). METHODS: The growth conditions of the cells were observed by soft agar colony formation assay, DNA laddering assay and flow cytometry assay after the gastric cancer cells were cultured in suspension. RESULTS : The MDCK cells as control group, which were derived from normal canine kidney, did not grow in soft agar and no cell colony was formed. The DNA ladder, the marker of apoptosis, appeared 15 h after the cell culture in suspension and the FCM assay showed that the cells had sub-G1 phase of cell cycle 24 h and 48 h after cell culture in suspension. But in the case of the other four gastric cancer cell lines (HGC27, etc), though different sizes of colon were observed when the cells were cultured in softer agar, there was no DNA ladder 15 h after the cell culture in suspension. By FCM assay, no significant difference was found between the cell cycle of suspensive cells and that of the control cells cultured in adhesion and no sub-G1 phase was observed. CONCLUSION: The MDCK cells are anchorage independent and the phenomena of anoikis can be observed. The four gastric cancer cells, HGC27, BGC823, MGC803 and SGC7901, are generally resistant to anoikis. ......
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