细胞周期蛋白类__(期刊论文)

过量胸苷对细胞周期素表达的影响

http://www.100md.com 《第四军医大学学报》 2003年第6期

细胞周期蛋白类,,胸苷；细胞周期蛋白类；肾小管；上皮细胞,0引言,1材料和方法,2结果,3讨论,【参考文献】

     (第四军医大学西京医院儿科，陕西西安 710033，邓州市第一人民医院儿科，河南邓州 474100，华西医科大学第二附属医院儿科，四川成都 610041)

    Effects of excessive thymidine on the expression of cyclin

    SHEN Qing, YANG CunMing, ZHANG GuoCheng, YAO YuJia, FU XueMei

    Department of Pediatrics, Xijing Hospital, Fourth Military Medical University, Xian 710033, China， Department of Pediatrics, First Peoples Hospital, Dengzhou City,Dengzhou 474100, China， Department of Pediatrics, Second Affiliated Hospital, Medical University of West China, Chengdu 610041,China

    【Abstract】 AIM： To explore the effects of excessive thymidine on the expression of cyclin A, B, D1 in each phases of cell cycle by studying the relationship between cyclin and DNA synthesis and the mechanism of cell cycle transition. METHODS： Neonatal pigs renal tubular epithelial cells were synchronized by the excessive thymidine block technique and the distribution of G1, S, G2 phases were determined by flow cytometry. The expression of cyclin A, B, D1 were detected by immunocytochemistry. RESULTS： Flow cytometry analysis revealed that (99.5±0.8)% of the neonatal renal tubular epithelial cells were at G1/S transition, (90.5±0.7)% in S phase and (93.5±2.2)% in G2 stage respectively by the method of the excessive thymidine block technique. The expression of cyclin A at G2 phases was higher than that of G1/S and S phases (average of gray level: 136.28±2.65, 145.04±1.35, 147.52±1.84 ......

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