大T抗原_____SV40；大T抗原；皮肤；成纤维细胞

SV40大T抗原对人皮肤成纤维细胞生物学行为的影响

http://www.100md.com 《第四军医大学学报》 2003年第6期

大T抗原,,SV40；大T抗原；皮肤；成纤维细胞,0引言,1材料和方法,2结果,3讨论,【参考文献】

     (第四军医大学口腔医学院病理学教研室，陕西西安 710033)

    Effects of SV40 large T gene transfection on biological behaviors of human skin fibroblasts

    WANG XinWen, JIN Yan, LIU Yuan, WANG JunLin, ZHAO Yu, DONG Rui

    Department of Pathology, Stomatological College, Fourth Military Medical University, Xian 710033, China

    【Abstract】 AIM： To investigate the role of SV40 large T (LT) antigen on human skin fibroblasts transformation and to observe the biological alteration of the cells. METHODS： Human skin firbroblasts were cultured and transfected with eukaryotic expressing plasmid psv3neo which containing the large T. After selection by G418 to stabilize the transfection, the large T mRNA and phenotypical changes of the transformants were examined. RESULTS： LT cDNA transformed cell colonies were isolated and were subcultured. LT mRNA expressed within the cytoplasm in most transformed cells when determined by in situ hybridization. After a period of time, however, the transfected cells gradually entered crisis and died. CONCLUSION： The ability of LT to enable the normal human skin fibroblasts immortalized is limited. So it is impossible to establish immortalized human skin fibroblast cell line only by large T. ......

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