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改进检测人类性染色质的方法
http://www.100md.com 《第四军医大学学报》 2004年第19期
性染色质,,性染色质;染液;检测方法,0引言,1材料和方法,2结果,3讨论,【参考文献】
     Improved detection method of human sex chromatin

    GU Wei,SUN Xian

    College of Life Science, Shaanxi Normal University, Xi’an 710062, China

    【Abstract】 AIM: To improve the detection method of human sex chromatin. METHODS: Human buccal mucosal cells were used as materials, the operation of centrifugal dropping slide was improved and the concentration levels were changed in three dyes, Thionin, Giemsa and Aceticgentian violet (AGV). Cover dyed method was employed in staining. RESULTS: Equalized Barr Body Frequency (BBF) was obtained in the samples that had been dyed respectively by Thionin, Giemsa and Aceticgentian violet (20.3±1.4)%, (19.5±1.5)% and (20.0±1.0)% respectively. CONCLUSION: The application of the improved detecting method of human sex chromatin enables us to detect sex chromatin immaculately.

    、【Keywords】 sex chromatin; dye; detection method

    【摘要】 目的:改进检测人类性染色质的方法. 方法:以人类口腔黏膜细胞为材料 ......

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