成纤维细胞生长因子_

sFGFR1基因的克隆与在RTS系统中的高效表达

http://www.100md.com 《第四军医大学学报》 2003年第8期

成纤维细胞生长因子,,受体，成纤维细胞生长因子；克隆，分子；RTS；基因表达,0引言,1材料和方法,2结果,3讨论,【参考文献】

     (第四军医大学：西京医院肝胆外科，科研部基因诊断技术研究所，陕西西安 710033)

    Cloning of sFGFR1 and its high efficient expression in RTS

    CHEN Yong, CUI DaXiang，SUN Kai,YANG YanLing,DAI Hui

    Department of Hepatobiliary Surgery, Xijing Hospital，Institute of Genetic Diagnosis Technology of Chinese PLA, Administration of Scientific Research, Fourth Military Medical University, Xian 710033,China

    【Abstract】AIM： To clone sFGFR1 gene and express corresponding protein in RTS. METHODS: Swiss rat 3T3 fibroblasts were cultured, total RNAs were extracted and sFGFR1 cDNA was obtained by RTPCR. The fragments were cut by means of NcoI and SmaI and products were cloned into pIVEX23 d vector and sequenced. SFGFR1 protein was expressed by using RTS ProteinMaster 500 and products were analyzed by Western Blot. RESULTS: sFGFR1 gene was cloned and sequencing analysis confirmed the cloned sequence was correct. Western Blot results confirmed sFGFR1 was highly expressed. CONCLUSION: sFGFR1 gene is cloned and highly expressed in RTS. ......

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