Effects of Chinese herb QDTMT on endothelial cells and PAIª²1 expression of atherosclerotic rat aorta

    WANG Zongª²Ren, ZHENG Jin, MA Aiª²Ling, Liu Qiang, MA Shiª²Ping, MA Jing, SU Mingª²Quan, LI Liª²Wen, LI Jingª²Hua

    1Department of Traditional Chinese Medicine, 3Department of Clinical Laboratory, Xijing Hospital, Fourth Military Medical University, Xi¬ðan 710033, China, 2Department of Hematology,Tangdu Hospital, Fourth Military Medical University, Xi¬ðan 710038, China

    ¡¾Abstract¡¿ AIM: To explore the effect of Chinese herb QiDanTongMai Tablet (QDTMT) on the rat artic endothelial cell lesion and the expression of PAIª²1 on aorta. METHODS: Seventyª²two male SD rats were divided into six experimental groups. Of the six groups, one was assigned randomly as the control group and was given normal diet (n=12) and one as the model group and was given vitamin D3, high fat and cholesterol diet to make atherosclerosis models (n=12). The other four groups, in addition to vitamin D3, high fat and cholesterol diet, were divided into low dose QDTMT group[0.36 g/(kg¡¤d)], moderate dose QDTMT group[1.08 g/(kg¡¤d)], high dose QDTMT group[3.24 g/(kg¡¤d)] and Simvastatin group[4 mg/(kg¡¤d)] for 16 weeks. The level of endothelial cell lesion was then observed by scanning electron microscope and the expression of PAIª²1 on aorta in every group was detected by reverse transcript polymerse chain reaction (RTª²PCR). RESULTS: The level of the endothelial cell lesion and the level of PAIª²1 expression in the model group were markedly higher than those of the control group. The significant decrease of PAIª²1 expression was detected in groups taking QDTMT. The higher the dose of the QDTMT, the lower the expression of PAIª²1. CONCLUSION: QDTMT can decrease the level of endothelial cell lesion and the expression of PAIª²1 on aorta in a doseª²dependent manner in rats with atherosclerosis. ......