神经胶质瘤_____tk基因；腺病毒；神经胶质瘤

腺病毒介导HSVtk/GCV系统杀伤脑胶质瘤C6细胞

http://www.100md.com 《第四军医大学学报》 2003年第7期

神经胶质瘤,,tk基因；腺病毒；神经胶质瘤,0引言,1材料和方法,2结果,3讨论,【参考文献】

     (沈阳军区总医院神经外科，辽宁沈阳 110015，吉林大学基础医学院病原生物学教研室，吉林长春130021)

    Killing effect of adenovirus mediated HSVtk/GCV system on glioma C6 cell

    XU ZaiHua, HUANG HongLan

    Department of Neurosurgery, General Hospital, Shenyang Military Region, Shenyang 110015, China, Department of Pathogenobiology, School of Basic Medical Sciences, Jilin University, Changchun 130021, China

    【Abstract】 AIM: To observe killing action of HSVtk/GCV system on glioma cells. METHODS: C6 cells were transfected by adenovirus with LacZ gene, then stained with Xgal for measuring the adenovirus transfected efficiency. C6 cells survival and bystander effect were observed after adding 10 mg·L-1 GCV to transfected cells. RESULTS: The more the multiplicity of infection (MOI), the more the adenovirus transfected efficiency to C6 cells (r=0.819, P<0.05). 100% transfected efficiency needed MOI 100. The killing action of HSVtk/GCV on C6 cells, depended on the amount of AdCMVtk (r=0.870, P<0.05). There was high bystander effect. CONCLUSION: Adenovirus mediated HSVtk/GCV system has good killing effect on glioma C6 cells. ......

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