破骨细胞__(期刊论文)

富含CpG的脱氧核苷酸片段对破骨细胞形成的调节作用

http://www.100md.com 《第四军医大学学报》 2004年第15期

破骨细胞,,破骨细胞；单核巨噬细胞集落刺激因子；细胞核因子受体活化子配基；肿瘤坏死因子α；CpG脱氧的核苷酸片段,0引言,1材料和方法,2

     CpG oligonucleotides in mediating osteoclast differentiation

    ZHAO WeiGong, HAN XueZhe, LIU Miao, GUO Xiong

    1Department of Orthopedics, First Hospital, 2Institute of Endemic Diseases, Medical School, Xi’an Jiaotong University, Xi’an 710061, China

    【Abstract】 AIM: CpG oligonucleotides were studied in their mediation of osteoclast differentiation. METHODS: Mouse bone marrow mononuclear cells were separated and simple bone marrow mononuclear cells or bone marrow mononuclear cells were incubated separately with 30 μg/L MCSF, 30 μg/L MCSF and 20 μg/L RANKL or with 30 μg/L MCSF and CpG ODN of different concentrations for 1-4 d. RESULTS: CpG ODN induced bone marrow mononuclear cells to differentiate into TRAPpositive osteoclasts and these cells expressed calcitonin receptors. Northern blot showed that the expression of TNFα mRNA increased with the increase of ODN from 5 nmol/L to 100 mol/L and it also increased gradually with prolonging time. CONCLUSION: CpG ODN stimulates the transformation of osteoclast, thus CpG ODN providing a potential therapeutic approach in treating bone diseases. ......

百拇医药网 http://www.100md.com/html/DirDu/2006/02/19/87/27/43.htm

您现在查看是摘要页，全文长 8798 字符。