一氧化氮_____一氧化氮；γ谷氨酰移换酶；精子

人精液中一氧化氮和γ谷氨酰基移换酶活性的关系

http://www.100md.com 《第四军医大学学报》 2004年第16期

一氧化氮,,一氧化氮；γ谷氨酰移换酶；精子,0引言,1材料和方法,2结果,3讨论,【参考文献】

     NO level and γglutamyltransferase activity of human sperm

    YANG MaiGui, YANG Yang, HAO XiaoKe, ZHANG ZhuYing, ZHENG ShanLuan, FAN AiLing, CHENG XiaoDong, ZHOU TieCheng

    1Department of Clinical Laboratories, Xijing Hospital, 2Cadet Brigade, Fourth Military Medical University, Xian 710033, China, 3Xian Hospital, General Company of Railway Construction, Xian 710016, China

    【Abstract】AIM: To study the relationship between the nitric oxide (NO) and the γglutamyltransferase (GGT) activity of human sperm. METHODS: Sperm routine analysis was conducted following WHO standard. Eightynine subjects were devided into 4 groups according to the density and activity of sperm. A coppered reduced cadmium fluometric assay was used to detect nitrate, a metablic product of nitric oxide in sperm. The activity of sperm GGT was detected by Glucana method. RESULTS: The content of NO had a significantly negative relationship with the density, the motility rate, and GGT activity of sperms in the 89 infertile patients (r =-0.56, -0.63, -0.71, P<0.01). CONCLUSION: The detection of the activity of sperm GGT is an effective index to assess the capability of sperms being fertilized. NO inhibits the activity of sperm GGT and affects the maturity and motility of sperms. ......

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