神经元_____低密度；细胞，培养的；神经元；形态学

低密度体外培养大鼠海马神经元的形态学观察

http://www.100md.com 《第四军医大学学报》 2003年第6期

神经元,,低密度；细胞，培养的；神经元；形态学,0引言,1材料和方法,2结果,3讨论,【参考文献】

     (第四军医大学全军神经科学研究所，陕西西安 710033)

    Morphological observation of rat hippocampal neuron in lowdensity culture in vitro

    SHEN JunXian, JIN WeiLin, JU Gong

    Institute of Neuroscience of Chinese PLA, Fourth Military Medical University, Xian 710033,China

    【Abstract】 AIM： To observe rat hippocampal neuron morphological characteristics at different developmental and differential stages in lowdensity culture. METHODS: Neuron and astrocyte threedimensional coculture method was used. Acquire newborn rat astroglial monolayer and dissect embryonic 18 d rat hippocampus and obtain singlecell suspension through trypsin digestion. Plate the suspension to polyLlysin coated coverglass in(2-5)×103 · cm-2 density. Transfer the coverglass to astrocyte culture section, coculture neurons with astrocytes in serumfree medium and observe neuronal morphological characteristics at different stages. RESULTS: Rat hippocampal neuron in lowdensity culture model was successfully acquired, which had distinct morphological characteristics at different stages. CONCLUSION: Hippocampal neuron in vitro has distinct morphological characteristics at different developmental and differential stages. ......

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