Effect of janus kinase/signal transducer and activator of transcription pathway on expression of tumor necrosis factorª²¦Á in liver of septic rats

    WANG Qiang, WANG Songª²Bai, DOU Keª²Feng, YAO Yongª²Ming

    1Department of Hepatobiliary Surgery, Xijing Hospital, Fourth Military Medical University, Xi¬ðan 710033, China, 2Department of Anesthesiology, PLA 251 Hospital, Zhangjiakou 075000, China, 3Institute of Burn, PLA 304 Hospital, Beijing 100037, China

    ¡¾Abstract¡¿ AIM: The purpose of the present study was to investigate the effects of janus kinase/signal transducer and activator of transcription (JAK/STAT) pathway on the expression of tumor necrosis factorª²¦Á(TNFª²¦Á) in hepatic tissues of rats with sepsis. METHODS: Using a sepsis model by cecal ligation puncture (CLP), 78 male Wistar rats were randomly divided into normal control group (n=6), CLP group (n=24), AG490 treatment group (n=24) and rapamycin (RPM) treatment group (n=24). At serial time points in each group, animals were sacrificed, hepatic tissue samples were harvested to determine TNFª²¦Á mRNA and protein expression levels. RESULTS: Compared with those of normal controls, the TNFª²¦Á mRNA levels significantly elevated in the liver 2-48 h after CLP (P<0.05) and the protein levels significantly increased at 2-48 h, respectively (P<0.05). Both treatment with either AG490 or RPM could markedly reduce hepatic TNFª²¦Á mRNA and protein expressions at 2, 6, 24 and 48 h following CLP (P<0.05). CONCLUSION: These data suggested that CLP sepsis challenge can increase TNFª²¦Á mRNA and protein expressions, which may be involved in JAK/STAT pathway. Inhibiting the activation of JAK/STAT pathway can reduce hepatic TNFª²¦Á mRNA and protein expressions, which may help to prevent the tissue injury after sepsis. ......