层粘连蛋白_____层粘连蛋白;胶原;神经元

基膜粘连蛋白和IV型胶原对培养脊髓神经细胞突起生长的作用

http://www.100md.com 《第四军医大学学报》 2003年第8期

层粘连蛋白,,层粘连蛋白;胶原;神经元,0引言,1材料和方法,2结果,3讨论,【参考文献】

     (第四军医大学西京医院整形外科, 陕西西安710033)

    Effects of laminin and type IV collagen on the axons growth of the cultured spinal neuron

    HAN Yan, LU KaiHua, YANG Li, ZHANG Hui

    Department of Plastic Surgery, Xijing Hospital, Fourth Military Medical University, Xian 710033, China

    【Abstract】AIM：To detect the influence of laminin and type IV collagen on the axon growth of the cultured spinal neuron. METHODS: Laminin and collagen IV were used in the experimental group and laminin and collagen IV combined with associated antibody, collagen I and polylysine were used in the control groups. Spinal neuron cells of the same density were cultured in each group. Each group was stained by the immunochemical method of Neuron-specific Enolase (NSE) and the length of the neuron axons was measured by the computer image system. RESULTS: The spinal neuron cells and their axons were stained by NSE. The axons length of the laminin and collagen IV groups was longer than that of other groups but no significant difference was observed. CONCLUSION: Laminin and collagen IV can stimulate the axon growth of spinal neuron cells. ......

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