杯状细胞__(期刊论文)

钙激活氯离子通道在气道杯状细胞合成黏蛋白中的促进作用

http://www.100md.com 《第四军医大学学报》 2004年第16期

杯状细胞,,哮喘；气道；上皮细胞；杯状细胞；氯化物通道,0引言,1材料和方法,2结果,3讨论,【参考文献】

     Calcium-activated chloride channel promotes synthesis of mucin in airway goblet cells

    SONG LiQiang, QI HaoWen, LI Yan, WANG JiCun, LIN AiHua

    1Department of Respiratory Medicine, 2Department of Cardiovasology, Xijing Hospital, 3Department of Biochemistry & Molecular Biology, School of Basic Medicine, 4Department of Outpatient, Fourth Military Medical University, Xian 710033, China

    【Abstract】 AIM: To investigate the effects of human calciumactivated chloride channel 1 (hCLCA1) on synthesis of mucin in airway goblet cells. METHODS: Recombined eukaryotic expression plasmid pIRES2EFGP/hCLCA1, which contained fluorescence tag and G418 screening flag was constructed. The plasmid was stably transfected into NCIH292 cell line, which was regarded as goblet cell model in vitro. Transfected NCIH292/hCLCA1 cells were identified by fluorescence microscopy and hCLCA1 mRNA was detected by RTPCR. Cells were divided into four groups with or without hCLCA1 inhibitor NFA: ① NCIH292; ② NCIH292/hCLCA1; ③ NCIH292+NFA; ④ NCIH292/hCLCA1+NFA. The proliferation of the cells in all groups was detected using MTT colorimetry. The expression of mucin MUC5AC protein and mRNA was examined with PAS staining and RTPCR assay, respectively. RESULTS: NCIH292/hCLCA1 cells that stably expressed hCLCA1 were obtained. The proliferation capability, mucin protein expression and mRNA level of NCIH292/hCLCA1 cells were all significantly higher than those of untransfected cells. NFA markedly inhibited the above changes of NCIH292/hCLCA1 cells. CONCLUSION: hCLCA1 promotes the proliferation and mucin synthesis in airway goblet cells. This study indicates that the inhibition of hCLCA1 may be a potential treatment for asthma. ......

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