〔收稿日期〕2003 —11 —05

    〔基金项目〕湖南省社会发展科技攻关计划基金资助项目[02 —96102(2) ]。

    〔作者简介〕周平兰(1971 —) ,女,湖南祁东人,主管药师,主要从事药品质量监测与检查。

    红花黄色素提取工艺的研究

    周平兰1

    , 夏新华2

    (1.湖南中医学院第二附属医院 , 湖南 长沙 410005 ; 2.湖南中医学院药学院 , 湖南 长沙 410007)

    〔摘要〕目的 探讨红花黄色素的最佳提取工艺。方法 考查浸泡时间、提取温度、提取次数、对红花黄色

    素提取的影响,采用紫外分光光度法测定,以红花黄色素的吸光度值为指标,考查回流法提取工艺条件,并

    用正交试验优选提取工艺条件。结果 浸泡时间、提取时间对红花黄色素的提取有非常显著性或显著性影

    响。结论 最佳提取工艺为红花用水提取2次,加水量为14、 12倍,浸泡时间为30 min ,提取时间为20 min。

    〔关键词〕红花; 红花黄色素; 正交试验; 提取工艺

    〔中图分类号〕R 284. 2 〔文献标识码〕A 〔文章编号〕1000 —5633(2004) 01 —0011 —02

    The Optimal Technology for the Extraction of Safflor Yellow

    Zhou Pinglan , Xia Xinhua

    (The Second Hospital , TCM Univ. of Hunan , Changsha , 410005)

    〔Abstract〕Objective To explore the optimal technology for the extraction of Safflor yellow(SY) .

    Methods Based on the orthogonal design , UV spectrophotometry was taken to determine the extracted con 2

    tent of SYwith the value of optical density as the index to evaluate the extracting conditions. In this way ,the

    effects of such conditions as soaking time , extracting times , extracting temperature and so on on SYcontent

    were investigated. Results The time of soaking and extracting gave very significant effects on the extracted

    content of SY. Conclusions The optimal technology for SY extraction is composed of two times of extracting

    with the added water value being 14 and 12 times that of materia medica ,30 min. of soaking period and 20

    min. of extracting time for each extraction respectively.

    〔Key words〕Carthamus tinctorius L. ; Safflor yellow; Orthogonal design ; Extracting technology

    红花( Carthamus tinctorius L. )为菊科植物 ,具有活血通

    经、祛瘀止痛的功效 ,是传统的活血化瘀中药。红花黄色素

    (Safflor yellow , SY )是含有多种有效成分的水溶性成分 ,被认

    为是红花的主要效应物质[1 ] ......