苦菜的组织培养与快速繁殖

    邱奉同, 张渝洁, 张 利 (临沂师范学院生命科学学院,山东临沂 276005)

    摘要 通过对苦菜的组织培养,研究了外植体不同取材部位及不同激素种类、浓度对愈伤组织及芽诱导和增殖的影响。结果表明:苦

    菜根的繁殖能力很强,但分化时间稍长;2 ,42D对愈伤组织的诱导能力较强,NAA对芽的诱导能力较强。同时,试验还筛选出了诱导愈

    伤组织的适宜培养基:MS + 62BA2. 0 mg L + 2 ,42D 0. 2 mg L ;芽分化培养基:MS + 62BA2. 0 mg L + NAA 0. 05 mg L ;继代培养基:MS + 62BA

    2. 0 mg L + NAA0. 2 mg L。

    关键词 苦菜;组织培养;快速繁殖

    中图分类号 S636. 9 文献标识码 A 文章编号 0517 - 6611(2005) 02 - 0241 - 02

    Tissue Culture and Rapid Propagation of Ixeris Chinensis

    QIU Feng2tong et al (Life Science Department , Linyi Teachers’University , Linyi ,Shandong 276005)

    Abstract The influence of different parts , different hormones and their concentrations on Ixeris Chinensi tissue culture inducing callus formation and

    bud growth was studied. The results showed that : during Ixeris Chinensis tissue culture , the root was easy to be induced , but it took more time to form

    bud. Through studying different hormones inducing buds and callus of Ixeris Chinensis ,we drew that : 2 ,42D induced callus more easily and NAA induced

    buds more easily. Simultaneously , in the experiment the appropriate media for the callus inducing was MS + 6 2BA 2. 0 mg L + 2 ,42D 0. 2 mg L ,buds

    differentiation and subculture had been picked :MS + 62BA2. 0 mg L + NAA0. 05 mg L ,MS + 62BA2. 0 mg L + NAA0. 2 mg L.

    Key words Ixeris Chinensis ;Tissue culture ;Rapid propagation

    苦菜[ Ixeris Chinensis (Thunb. ) Nakai ]是菊科苦菜属1 年

    生或多年生草本植物,别名清苦菜、苦荬菜。随着苦菜应用

    价值的不断开发,其栽培技术也迅速发展起来。目前,苦菜

    的繁殖主要有种子和根状茎2 种方式。关于植物组织培养

    技术在苦菜的繁殖和育种上的应用尚未见报道。通过组织

    培养诱导的愈伤组织和丛生芽,可以高效地诱导突变,改良

    品种,加速苦菜栽培品种的育成。为此,笔者对苦菜的组织

    培养和快速繁殖技术进行了研究。

    1 材料与方法

    1. 1 材料 野生苦菜全株,采自山东临沂 河河岸。

    1. 2 培养基 以MS为基本培养基,pH值5. 8～6. 0 ,琼脂6. 8

    gL ,诱导培养基附加蔗糖30 g L ,生根培养基附加蔗糖15

    g L。 诱导培养基: ① MS + 62BA(0. 5 , 1. 0 , 2. 0 ,2. 5) mg L +

    NAA(0 ,0. 01 ,0. 05 ,0. 10 ,0. 20 , 0. 50) mg L ; ② MS + 62BA(0. 5 ,1. 0 ,2. 0 ,2. 5) mg L + 2 ,42D(0 ,0. 01 ,0. 05 ,0. 10 ,0. 20 ,0. 50)

    mgL。继代培养基: ③ MS + 62BA(0. 5～2. 0) mg L + NAA 0. 2

    mg L ; ④ MS + KT(0. 5～2. 0) mg L +NAA0. 2mg L ......