利用大鼠Y染色体特异性PCR技术检测卵圆细胞源性肝癌细胞
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陈铁军, 方驰华, 朱明德
干细胞;肝卵圆细胞;肝干细胞;免疫组织化学,陈铁军,方驰华,朱明德,陈铁军,通讯作者:,Detectionoflivercancercel
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陈铁军, 方驰华, 朱明德, 南方医科大学珠江医院肝胆外科 广东省广州市 510282
陈铁军, 男, 1979-03-05生, 辽宁省鞍山市人, 汉族, 2003年南方医科大学硕士生, 主要从事肝胆胰外科疾病的研究.
广东省自然科学基金资助项目, No. 2002.020097
通讯作者: 方驰华, 510282, 广东省广州市, 南方医科大学珠江医院普外科. fch58520@sina.com
电话: 020-35509612
收稿日期: 2005-11-23 接受日期: 2005-12-02
Detection of liver cancer cells originated from hepatic oval cells using rats’ Y chromosome specific polymerase chain reaction technique
Tie-Jun Chen, Chi-Hua Fang, Ming-De Zhu
Tie-Jun Chen, Chi-Hua Fang, Ming-De Zhu, Department of Hepatobiliary Surgery, Zhujiang Hospital of Southern Medical University, Guangdong 510282, Guangdong Province, China
Supported by the Natural Science Foundation of Guangdong Province, No. 2002.020097
Correspondence to: Chi-Hua Fang, Department of General Surgery, Zhujiang Hospital of Southern Medical University, Guangdong 510282, Guangdong Province, China. fch58520@sina.com
Received: 2005-11-23 Accepted: 2005-12-02
Abstract
AIM: To find out the evidence that hepatic oval cells could differentiated into liver cancer cells, and to provide the theoretical basis for clinical therapy of liver cancer.
METHODS: Thirty male Wistar rats were fed with the animal food containing 0.6 g 3’3-diaminobenzidine (3’-Me-DAB) per kilogram for 4 weeks to establish the model of hepatic oval cell proliferation. Then the hepatic oval cells were extracted and identified by immunohistochemistry under light and electron microscope. Sixty female Wistar rats were randomly and averagely divided into experiment group and control group. The rats in the control group were fed with the DAB-containing food for 14 weeks without transplantation. While the rats in experiment group were narcotized with 25 mg/kg nembutal via abdominal cavity, and the male hepatic oval cells (in suspension) were inoculated (106 each point) under the liver envelope after the abdominal part was cut open ......
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