树突状细胞和调节性T细胞在自身抗原所致小鼠免疫耐受模型中的作用
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向 明, 张程亮, 邹晓蕾, 蔡晓寒, 彭佳蓓
自身抗原;树突状细胞;调节性T细胞;1型糖尿病,向明,张程亮,邹晓蕾,蔡晓寒,彭佳蓓,向明,通讯作者:,Rolesofdendrit
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向明, 张程亮, 邹晓蕾, 蔡晓寒, 彭佳蓓, 华中科技大学同济医学院药学院药理教研室 湖北省武汉市 430030
向明, 女, 湖南溆浦人, 华中科技大学同济医学院药学院药理教研室副教授, 博士, 主要从事免疫药理学即自身免疫病发病机制和治疗方法研究.
国家自然科学基金资助项目, No.30200343
通讯作者: 向明, 430030, 湖北省武汉市, 华中科技大学同济医学院药学院药理教研室. xiangming@mails.tjmu.edu.cn
电话: 027-83657813 传真: 027-83692892
收稿日期: 2006-01-10 接受日期: 2006-02-08
Roles of dendritic cells and regulatory T cells in autoantigen-induced murine immune tolerance model
Ming Xiang, Cheng-Liang Zhang, Xiao-Lei Zou, Xiao-Han Cai, Jia-Bei Peng
Ming Xiang, Cheng-Liang Zhang, Xiao-Lei Zou, Xiao-Han Cai, Jia-Bei Peng, Department of Pharmacology, College of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China
Supported by National Natural Science Foundation of China, No. 30200343
Correspondence to: Ming Xiang, Department of Pharmacology, College of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China. xiangming@mails.tjmu.edu.cn
Received: 2006-01-10 Accepted: 2006-02-08
Abstract
AIM: To investigate the important roles of dendritic cells (DC) and CD4+CD25+ regulatory T cells in immune prevention against insulin dependent diabetes (IDDM) by autoantigen insulin administration.
METHODS: The model of IDDM was established by intraperitoneal injection of low-dose streptozotocin (STZ) 40 mg/kg per day for 5 consecutive days in Balb/c mice. The bovine insulin (100 μg) in incomplete Freund's adjuvant (IFA, emulsified 1∶1) was given subcutaneously to the mice weekly for 4 weeks. The blood glucose was examined once a week and all the mice were killed after 5 weeks. Pancreas tissues were collected for histopathological examination. DC precursor cells from bone marrow and lymphocytes from spleen were isolated. The phenotype of DC and CD4+ CD25+ regulatory T cells were analyzed by fluorescence activated cell sorter (FACS) ......
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