肝包虫周围纤维囊壁中骨桥蛋白免疫印记与组化分析
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李建辉, 彭心宇, 唐 娟, 吴向未, 张示杰,
肝;细粒棘球蚴;骨桥蛋白;免疫印记,李建辉,彭心宇,吴向未,张示杰,牛建华,孙红,牛少雄,吕游,唐娟,李建辉,通讯作者:,Analysisofos
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李建辉, 彭心宇, 吴向未, 张示杰, 牛建华, 孙红, 牛少雄, 吕游, 石河子大学医学院第一附属医院普外一科 新疆维吾尔族自治区石河子市 832008
唐娟, 新疆石河子大学医学院组胚教研室 新疆维吾尔族自治区石河子市 832002
李建辉, 男, 1980-06-21生, 山西太原人, 汉族, 石河子大学医学院附属第一医院普外一科在读硕士研究生, 主要从事肝脏外科研究.
国家自然科学基金攻关项目, No.30260108
教育部科学技术研究重点项目, No.03162
通讯作者: 彭心宇, 832008, 新疆维吾尔族自治区, 石河子大学医学院第一附属医院普外一科. pengxinyu2000@sina.com.cn
电话: 0993-2858842 传真:0993-2858842
收稿日期: 2005-12-23 接受日期: 2006-01-14
Analysis of osteopontin in pericystic layer of hepatic hydatid cyst by Western blotting and immunohistochemical technique
Jian-Hui Li, Xin-Yu Peng, Juan Tang, Xiang-Wei Wu, Shi-Jie Zhang, Jian-Hua Niu, Hong Sun, Shao-Xiong Niu, You Lv
Jian-Hui Li, Xin-Yu Peng, Xiang-Wei Wu, Shi-Jie Zhang, Jian-Hua Niu, Hong Sun, Shao-Xiong Niu, You Lv, Department of General Surgery, the First Affiliated Hospital, Medical College of Shihezi University, Shihezi 832008, Xinjiang Uygur Autonomous Region, China
Juan Tang, Department of histoembryology, Medical College of Shihezi University, Shihezi 832008, Xinjiang Uygur Autonomous Region, China
Supported by National Natural Science Foundation of China, No.30260108
Correspondence to: the First Department of General Surgery, the First Affiliated Hospital, Medical College of Shihezi University, Shihezi 832008, Xinjiang Uygur Autonomous Region, China. pengxinyu2000@sina.com.cn
Received: 2005-12-23 Accepted: 2006-01-14
Abstract
AIM: To investigate the expression and distribution of osteopontin (OPN) in hepatic hydatid cyst and its significances.
METHODS: Proteins extracted from 60 surgically excised hydatid cyst tissues were subjected to polyacrylamide gel electrophoresis followed by Western blotting, using polyclonal antibodies directed against OPN; the distribution of OPN was also observed by immunohistochemical techniques.
RESULTS: OPN expression was detected in 48(80%) cases of cysts by Western blotting ......
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