VEGF基因表达抑制对胃腺癌细胞SGC-7901增殖的影响
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徐文华, 葛银林, 徐宏伟, 王秀丽, 耿芳宋
胃癌;SGC-7901;RNA干扰;血管内皮细胞生长因子,徐文华,葛银林,徐宏伟,王秀丽,耿芳宋,徐文华,通讯作者:,Effectofd
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徐文华, 葛银林, 徐宏伟, 王秀丽, 耿芳宋, 青岛大学医学院生物化学与分子生物学教研室 山东省青岛市 266021
徐文华, 女, 1972-09-15生, 山东省青岛市人, 汉族, 青岛大学医学院硕士研究生.
青岛市科技局科技计划项目, 03-1-NY-14
通讯作者: 葛银林, 266021, 山东省青岛市登州路38号, 青岛大学医学院生物化学与分子生物学教研室. geyinlin@126.com
电话: 0532-82991209 传真: 0532-82991009
收稿日期: 2005-12-28 接受日期: 2006-02-10
Effect of down-regulating vascular endothelial growth factor gene on proliferation of gastric cancer cell line SGC-7901
Wen-Hua Xu, Yin-Lin Ge, Hong-Wei Xu, Xiu-Li Wang, Fang-Song Geng
Wen-Hua Xu, Yin-Lin Ge, Hong-Wei Xu, Xiu-Li Wang, Fang-Song Geng, Department of Biochemistry and Molecular Biology, Medical College of Qingdao University, Qingdao 266021, Shangdong Province, China
Supported by Science Project of Qingdao Science and Technology Administration, No. 03-1-YN -14
Correspondence to: Professor Yin-Lin Ge, Department of Biochemistry and Molecular Biology, Medical College of Qingdao University, Qingdao 266021, Shandong Province, China. geyinlin@126.com
Received: 2005-12-28 Accepted: 2006-02-10
Abstract
AIM: To study the effect of small interfering RNA (siRNA) on the gene expression of vascular endothelial growth factor (VEGF) as well as the proliferation of gastric cancer cell line SGC-7901.
Two groups of siRNA were designed targeting VEGF mRNA and finally obtained by in vitro transcription. Then the obtained siRNA was transfected into cultured human gastric cancer cell line SGC-7901 by Lipofectamine. Hoechst33258 staining was used to observe the apoptotic bodies in the cells, and flow cytometry was used to detect the change of cell cycles. The level of VEGF mRNA expression was analyzed by reverse transcription polymerase chain reaction (RT-PCR) and the secretion of VEGF protein in the supernatant was examined by enzyme-linked immunosorbent assay (ELISA).
RESULTS: Both groups of VEGF-targeted siRNA inhibited the proliferation of SGC-7901 cells and induced cell apoptosis effectively and after transfection ......
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