关键词: 降钙素基因相关肽;成骨细胞;破骨细胞;白细胞介素-1

    摘 要:目的 了解白细胞介素-1β(IL-1β)在破骨细胞性骨吸收中的刺激作用，并评价降钙素基因相关肽(CGRP)的拮抗效果. 方法 将新生大鼠破骨细胞和成骨细胞混合培养，接种于预置象牙片的培养板中.24h后培养液中分别加入IL-1β和不同浓度的CGRP.继续培养48h，然后取出象牙片，超声处理后行甲苯胺蓝染色，光镜下观察骨吸收陷窝数目并计算其总面积. 结果 IL-1β明显增加象牙片上吸收陷窝的数目(37.2±8.2vs22.4±5.7)per slice(P<0.01)和面积(μm2 ，9761±2775vs4224±1381，P<0.01)，而CGRP则能够显著抑制IL-1β的刺激作用(P<0.05或P<0.01)，且抑制作用呈剂量依赖性(r1 =-0.56，P<0.01;r2 =-0.49，P<0.05). 结论 CGRP可直接作用于破骨细胞并抑制其活化，并调节成骨细胞相关因子的释放而间接影响破骨细胞功能.

    Calcitonin gene┐related peptide inhibits bone resorp┐tion mediated by interleukin┐1in rats

    LIAN Kai DUJing-Yuan RAO Zhen-Yu LUO Huai-Can

    1 Institute of Orthopaedics of Chinese PLA，Xijing Hospital，Fourth Military Medical University，Xi'an710033，China，

    2 Department of Orthopaedics，Xiehe Hospital，Tongji Medical University，Wuhan430022，China

    Keywords:calcitonin gene-related peptide;osteoblast;osteo-clast;interleukin-1

    Abstract:AIM To investigate the effects of interleukin-1β(IL-1β)on bone resorption in vitro，and to evaluate the inhi-bitory effect of CGRP.METHODS The osteoclasts isolated from the long bones of newborn SD rats were co-cultured with osteoblasts on ivory slices placed in24-well plates.Twenty-four hours later ......