Changes of hydroxyproline and oxidativeª²stress indices in bleomycinª²induced pulmonary fibrosis in rats

    LIU Rui, LI Chengª²Gang, LIANG Xin, HAI Chunª²Xu

    Department of Toxicology, School of Preventive Medicine, Fourth Military Medical University, Xi¡¯an 710033, China

    ¡¾Abstract¡¿ AIM: To discuss the changes of hydroxyproline and the role of oxidationª²antioxidation disequilibrium in pulmonary fibrosis induced by bleomycin (BLM) in rats. METHODS: A single dose BLM was intratracheally injected to induce pulmonary fibrosis in rats, so as to observe the changes of tissue hydroxyproline and oxidativeª²stress markers during the process. The animals were killed on the 3rd, 7th, 14th and 28th d separately. The pathological model was evaluated by HE and Masson dyeing method, and the hydroxyproline of lung tissue was determined. At the same time, malonaldehyde (MDA) content, superoxide dismutase (SOD) activity, reduced glutathione/oxidized glutathione (GSH/GSSG) ratio of lung tissue were also detected. RESULTS: From 7 d to 28 d, the hydroxyproline of lung tissue was increased in BLM groups compared with that in control groups, especially on the 28th d (P<0.05). On the 7 d and14 d, the tissue MDA were enhanced while the activity of SOD was decreased compared with those of control (P<0.05). The GSH/GSSG ratio was lower than that of control on the 3 d and 7 d (P<0.05). CONCLUSION: Tissue hydroxyproline can be a marker of BLMª²induced pulmonary fibrosis. The oxidationª²antioxidation disequilibrium of cells and matrix may play a role in the pathological process. ......