Thrombospondinª²1, microvessel density in primary nonª²small cell lung cancer and their clinical relevance

    DONG Jiª²Min, HU Xiaoª²Rong, SUN Aiª²Xiong£¬HE Jiª²Yin, LI Xiuª²Xia

    1Department of Geriatrics, Xi¬ðan Centric Hospital, Xi¬ðan 710003, China, 2Department of Obstetrics & Gynecology, Hospital of Workers & Staffs of Yanan Cigarette Factory, Yanan 716000, China, 3Family Planning Instructory Station of Zichang County, Zichang 717300, China, 4Department of Respiratory Medicine,Second Hospital, Medical College,Xi¬ðan Jiaotong University, Xi¬ðan 710003, China

    ¡¾Abstract¡¿AIM£º To explore the relationship between the expression of thrombospondin (TSP) and angiogenesis in nonª²small cell lung cancer (NSCLC). METHODS: By using SP immunohistochemistry stainning method, expression of TSP1 and TSP2 and microvessel counting were evaluated in surgically resected specimens from 55 patients with NSCLC and 30 adjacent normal tissues using the antiª²TSP1, antiª²TSP2 and antiª²CD34 monoclonal antibodies, respectively. RESULTS: Active angiogenesis was observed in NSCLC tumor tissues. The microvessel density (MVD) was related to metastasis and the clinical stage of NSCLC. TSP1, TSP2 expressed mainly in the cytoplasm, nuclear and extracellular matrix. The expression of TSP1 and TSP2 in the 55 tumor tissues was significantly higher than that in the 30 adjacent normal tissues(158.70 ¡À 2ª±11 versus 141ª±20¡À7.20 and 149.10¡À2.94 versus 145.70¡À[yn1]4.74, respectively, P<0.05). The expression of TSP1 and TSP2 in NSCLC patients was related to the clinical stage and metastasis of NSCLC. There was a negative correlation between TSP1, TSP2 expression and MVD (r£½-0.964,-0.876, respectively, P<0.05). TSP1 played a more important role. CONCLUSION: It indicates that TSP1 and TSP2 are closely correlated to the angiogenesis of NSCLC. TSP1 and TSP2, together with MVD, may serve as clinical predictors for the metastasis of NSCLC. ......