关键词: 应激;下丘脑;血管紧张素原mRNA;原位杂交

    摘 要:目的 观察应激时大鼠下丘脑血管紧张素原mRNA表达及血管紧张素Ⅱ含量的变化. 方法 雄性大鼠48只随机分为3组，游泳组予以4℃～6℃冷水游泳刺激;电击组予以0.5～2Hz，60～100V足底电刺激，每日2次，每次2h，持续1wk;对照组不予任何刺激.用原位杂交技术及放免分析法检测不同应激时大鼠下丘脑血管紧张素原(Ang)mRNA的表达及血管紧张素Ⅱ(AngⅡ)含量的变化. 结果 应激大鼠下丘脑Ang mRNA阳性表达面积百分比与积分吸光度均显著增高.游泳组(2.48±0.40)%与(0.75±0.09)，电击组(2.94±0.82)%与(1.83±0.41)vs对照组(0.20±0.04)%与(0.24±0.12)，P<0.01;AngⅡ含量也显著增高.游泳组(141±67)ng g

    -1 ，电击组(84±25)ng g-1 vs对照组(56±16)ng g-1 ，P<0.01，P<0.05，且Ang mRNA表达增高率(1216±196)%和(1441±402)%，超过了AngⅡ含量的增高率(295±118)%和(149±44)%，P<0.01. 结论 应激时下丘脑肾素-血管紧张素系统(RAS)被显著激活，进一步支持局部RAS参与了应激反应的观点.

    Expression of angiotensinogen mRNA in rat hy┐pothalamus during stress

    XU Yong ZHANG Su-Zhen WAN Yu YANG Gang

    1 Department of Physiology，

    2 Department of Patho-physiology，Guangdong Medical College，Zhanjiang524023，China，

    3 Department of Physiology，Medi-cal College of Wuhan University，Wuhan430071，China

    Keywords:stress;hypothalamus;angiotensinogen mRNA;in situ hybridization

    Abstract:AIM To investigate the expression of an-giotensinogen(Ang)mRNA and the changes of angiotensinⅡ(AngⅡ)contents in rat hypothalamus during stress.METHODS Forty-eight male rats were randomly allocated to three groups.Swimming rats were treated with swimming stimulation in4℃～6℃cold water;electric shock rats were treated with repetitive0.5～2Hz ......