关键词: GABA;甘氨酸;延髓;免疫电镜;大鼠

    摘 要:目的 观察大鼠延髓背角(MDH，三叉神经脊束核尾侧亚核)浅层(Ⅰ，Ⅱ层)内降钙素基因相关肽(CGRP)阳性终末与γ-氨基丁酸(GABA)能和甘氨酸(Gly)能神经元的突触联系. 方法 包埋前GABA或Gly免疫组织化学染色方法结合包埋前CGRP免疫金标记电镜技术. 结果 GABA或Gly阳性产物广泛分布于神经元胞体、轴突和树突内，CGRP阳性标记主要见于无髓和薄髓轴突内.约53%和51%的CGRP阳性轴突终末分别与GABA和Gly阳性神经元的胞体或树突形成非对称性突触，其中的多数为轴-树突触，轴-体突触较少. 结论 含CGRP的初级传入纤维直接与MDH浅层内的GABA能及Gly能抑制性中间神经元形成突触联系.

    GABA┐and glycine┐immunoreactive neurons postsy┐naptic to calcitonin gene┐related peptide┐immunore┐active terminals in the superficial layers of the rat medullary dorsal horn

    FENG Yu-Peng，YANG Kun，LI Yun-Qing

    Department of Anatomy and K.K.Leung Brain Re-search Centre，The Fourth Military Medical Uni-versity，Xi'an710033，China

    Keywords:GABA;glycine;medulla oblongata;microscope，immunoelectron;rats

    Abstract:AIM To investigate the synaptic connections be-tween calcitonin gene-related peptide-immunoreactive(CGRP-ir)terminals andγ-aminobutyric acid(GABA)-and glycine(Gly)-ir neurons in the superficial layers(laminae I and II)of rat medullary dorsal horn(MDH).METHODS Pre-embedding immunohistochemical detection of GABA or Gly ......