关键词: 微核;胸腔积液;染色体，人;染色体畸变

    摘 要:目的 探讨胸水中淋巴细胞微核率(PFLMNF)对良、恶性胸腔积液鉴别诊断的意义. 方法 对良、恶性胸腔积液患者及健康人各20例采用细胞分裂阻滞微核(CB-MN)法检测外周血淋巴细胞微核率(PBLMNF)及用丝裂霉素C(MMC)诱发PBLMNF，直接用光镜观察PFLMNF. 结果 PFLMNF与PBLMNF有良好的相关性(r=0.935，P<0.01).恶性胸腔积液患者自发PBLMNF及诱发PBLMNF均显著高于对照组和良性胸腔积液组(P<0.01).良性胸腔积液组PBLMNF与对照组无统计差异(P>0.05).恶性胸腔积液组PFLMNF较良性胸腔积液组明显升高(P<0.01). 结论 PFLMNF检测方法简单、快速、实用，并可以代替PBLMNF检测，PFLMNF检测可成为良、恶性胸腔积液鉴别诊断的一种有实用价值的方法.

    Significance of lymphocyte micronucleus test in pleural fluid for diagnosis of malignant pleural ef┐fusion and benign pleural effusion

    FENG En-Zhi，QI Hao-Wen，XUE Jun

    Department of respiratory disease，Xijing Hospital，Fourth Military Medical University，Xi'an710033，China

    Keywords:micronuclei;pleural effusion;chromosomes，hu-man;chromosome aberrations

    Abstract:AIM To investigate the significance of pleural fluid lymphocyte micronuclei frequency(PFLMNF)for diag-nosis of malignant pleural effusion and benign pleural effu┐sion.METHODS The modified cytokinesis-blocked mi-cronucleus(CB-MN)technique was used to determine pe-ripheral blood lymphocyte micronuclei frequency(PBLMNF)and mitomycin C(MMC)-induced PBLMNF in20cases with benign pleural effusion ......