关键词: 人;L1基因;克隆;人乳头瘤病毒;宫颈癌

    摘 要:目的 从已感染人乳头瘤病毒(HPV)的新鲜宫颈癌组织中获得HPV16型晚期表达基因L1，为HPV感染的检测和治疗奠定基础. 方法 根据引物设计原则设计一对特异引物，并用PCR方法从宫颈癌组织中获得L1基因，用pGEM-T作为克隆载体，构建重组质粒并以双脱氧法双向测定插入片段序列，拼接出L1基因序列，自动测序验证并分析序列. 结果 从西安地区1例宫颈癌临床标本克隆到的1株HPV16型L1蛋白编码序列经BLAST2.0分析与既往报道序列存在高度同源性. 结论 构建的重组质粒为进一步研究L1蛋白的表达及其免疫学创造了条件.

    Cloning and sequence analysis of L1major capsid protein gene of human papillomavirus type16

    CHEN Rui ZHANG Ju YAN Xiao-Jun LI Qing LI Ding

    1 Institute of Gene Diagnosis of Chinese PLA，

    2 Department of Pathology，Faculty of Preclinical Medicine，Fourth Military Medical University，Xi'an710033，China

    Keywords:human;L1gene;cloning;human papillo-mavirus;cervical carcinoma

    Abstract:AIM To obtain the L1major capsid protein gene of human papillomavirus type16for the exploring of the clin-ical analysis and treatment of HPV.METHODS L1gene was amplified from human cervical carcinoma by RT-PCR.The product of PCR was ligated into pGEM-T vector and used to transform E.coli JM-109.Following blue and white selection screening ......