关键词: 钙/分析;局部缺血;氟桂嗪

    摘 要:目的 研究类缺血状态下神经元胞质内游离钙离子及细胞活性变化，探讨缺血性神经元的损伤与细胞内钙离子的关系及西比灵的治疗意义. 方法 利用Ca2+ 指示剂Flu-3/AM作为细胞内钙离子的荧光探针负载培养的神经元，共聚焦技术检测细胞内荧光强度的变化，利用四唑蓝(MTT)比色试验检测缺血性神经元的损伤程度. 结果 给予神经元类缺血处理10min后，缺血组神经元胞质内钙离子浓度和细胞损伤程度明显高于西比灵预处理组(P<0.01). 结论 西比灵可明显抑制神经元胞质内钙离子的升高，减轻由此引发的缺血性神经元的损伤程度.

    Effects of Sibilium on intracellular Ca2+ and cell ac┐tivity in cortical neurons on ischemic┐like condition

    LIU Yong-Hong，WAN Qi，ZHANG Wei，LI Li，WANG Hong-Dian

    Department of Neurology，Xijing Hospital，Fourth Military Medical University，Xi'an710033，China

    Keywords:calcium/analysis;ischemia;flunarizine

    Abstract:AIM To study the changes of intracellular Ca2+ and cell activity and protection of Sibelium on cortical neu-rons on ischemic-like condition.METHODS Neurons were cultured and loaded with Flu-3/AM，[Ca2+ ]I was measured by fluorescent intensity(FI)in each cell with confocal mi-croscopy，and the extent of neurons injury was analyzed with MTT reduction assay.RESULTS After10min ischemic-like conditioning ......