关键词: 高效液相色谱;海马;天门冬氨酸;谷氨酸;牛磺酸;γ-氨基丁酸

    摘 要:目的 建立一种可同时测定微量谷氨酸(Glu)，天门冬氨酸(Asp)，牛磺酸(Tau)，γ-氨基丁酸(GABA)4种氨基酸类神经递质的HPLC分析方法. 方法 制备大鼠海马脑片，灌流取样，冻干再溶后，采用邻苯二甲醛(OPA)柱前衍生，反相梯度洗脱的高效液相/荧光检测法，测定其中4种微量氨基酸的含量. 结果 各氨基酸的浓度与峰面积均呈现良好的线性关系，相关系数(r)分别为:Asp0.9920，Glu0.9667，Tau0.9803和GABA0.9584;日内变异系数(%)为Glu4.3，Asp4.6，Tau6.7和GABA7.1;最低检出限(pmol L-1 )为:Asp0.17，Glu0.35，Tau0.68和GABA1.12，标准加入回收率(%)分别为:Asp106±7，Glu101±5，Tau94±6和GABA92±11. 结论 该法具有衍生化反应操作简便，流动相成本低，灵敏度高，特异性强，分析时间短的优点，而且同样适用于其他生物样品的测定.

    Determination of amino acid neurotransmitters in the hippocampal slices perfusate by RP┐HPLC

    WANG Xiao-Fei LUO Xiao-Xing ZHOU Si-Yuan ZHAO De-Hua CHENG Tao PAN Xiao-Jun

    1 Department of Pharmacology，Faculty of Preclini-cal Medicine，Fourth Military Medical University，Xi'an710033，China，

    2 Department of Anesthesiolo-gy，Qilu Hospital，Shandong Medical University，Ji'nan250012，China

    Keywords:HPLC;hippocampus;aspartic acid;glulamate;taurine;GABA

    Abstract:AIM To establish a method for simultaneous de-termination of trace amino acid neu

    rotransmitters Glu，Asp，Tau and GABA by HPLC was improved.METHODS The sample prepared by rat hippocampal slices perfusation was lyophi-lized and resoluted ......