关键词: 生物医学工程;聚羟基乙酸;成骨细胞;胶原;骨形态发生蛋白质类

    摘 要:目的 探讨组织工程化骨修复兔颅骨缺损的实用价值及合理应用BMP的方法. 方法 将自体成骨样细胞即刻种植在复合BMP的胶原包埋的聚羟基乙酸(PGA)的基质材料和不含BMP的基质材料上，然后将不同的复合体或单纯基质材料移植到兔颅骨的一侧全层骨缺损区，作为实验侧I、实验侧Ⅱ或实验侧Ⅲ.对侧设对照，不作任何植入.将60只新西兰兔分别于术后2，6，8及12wk处死，标本行大体组织学检查，X线摄片及灰量测定检查. 结果 表明实验侧I成骨量明显大于实验侧Ⅱ或实验侧Ⅲ(P<0.01)，而对照侧仅为纤维组织修复. 结论 组织工程骨可修复自体的兔颅骨缺损，应用外源性BMP可促进骨再生，为组织工程化骨在颅面外科及整形外科领域的临床应用奠定了基础.

    Repair of rabbit calvarial defects using the tissue engineered bone compounded with BMP

    WANG Yang AI Yu-Feng LIOU Da-Lie XONG Men GUO Shu-Zong

    1 Department of Plastic Surgery，General Hospital of Shenyang Militery Region，Shenyang110015，Chi-na.

    2 Department of Plastic Surgery，Fourth Mili-tary Medical University，Xi'an110073，China

    Keywords:biomedical engineering;polyglycolic acid;os-teoblasts;collagen;bone morphogenetic proteins

    Abstract:AIM To explore the practical value of repairing the defects of rabbit calvarial with tissue engineering tech-nique and the way of rational application of BMP.METHODS The osteoblast-like cells were instantly implanted onto the BMP compounded polyglycolic acid(PGA)matrix coated with collagen and pure PGA matrix coated with collagen.60NewZealand rabbits were divided into three groups random-ly.In Group One ......