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编号:10929068
贫铀对人肾小管上皮细胞的恶性转化及苯乙酸、亚硒酸钠的抑制作用
http://www.100md.com 《第四军医大学学报》 2004年第20期
亚硒酸钠,,贫铀;恶性表型;苯乙酸;亚硒酸钠,0引言,1材料和方法,2结果,3讨论,【参考文献】
     Transformation of human kidney cells to tumorigenic phenotype by depleted uranium and suppression by phenyl acetate and sodium selenite

    LI Rong, AI GuoPing, XU Hui, LOU ShuFen, CHENG TianMin, SU YongPing, ZHENG HuaiEn, JIANG JianXin, HUANG YueSheng

    State Key Laboratory of Trauma, Burn & Combined Injury, Institute of Combined Injury, College of Military Preventive Medicine, Third Military Medical University, Chongqing 400038, China

    【Abstract】 AIM: To investigate whether depleted uranium (DU) could transform human kidney cells (HKC) to tumorigenic phenotype and whether antitumor inhibitor could be used as an effective measure to prevent carcinogenesis. METHODS: After 24 h exposure to soluble DU (0.063-0.500 g/L), HKCs were incubated for 1 day to 3 months with or without 1.0-2.5 μmol/L phenyl acetate or sodium selenite. The colony form efficient (CFE), growth curve and ConA agglomeration test in cells were observed. Some experiments such as anchorageindependent growth, tumor formation in nude mice, production of fragile histidine triad (FHIT)tumorsuppressor protein in cells were conducted. RESULTS: From 20 to 30 passages, HKC induced by DU formed colonies in soft agar and produced tumor in athymic mice. Expression of FHIT protein in some cells decreased. Both phenyl acetate and sodium selenite reduced the frequency of colony formation in soft agar to (0.6±0.1)‰. No tumor was found in athymic mice (0/3) (P<0.05). CONCLUSION: HKCs treated with DU solution are transformed into tumorigenic phenotype. The transformation in vitro can be suppressed effectively by phenyl acetate and sodium selenite. ......

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