摘要：目的：探讨幽门螺杆菌粪便抗原(Helicobacter pylori stool antigen，HpSA)检测在诊断儿童和老年患者Hp感染的应用价值。方法：采用ELISA(enzyme-linked immunosorbent assay，ELISA)法检测30例和48例因上消化道症状接受胃镜检查的儿童和老年患者的粪便标本(以快速尿素酶试验、细菌培养和组织学Warthin-Starry染色作为诊断Hp的“金标准”，3项检测中有2项试验阳性则诊断为Hp感染，3项检测均阴性则诊断为Hp阴性)。结果：在30例儿童和48例老年患者中，“金标准”诊断Hp感染阳性43例，阴性35例；“金标准”诊断43例阳性中HpSA检测有40例阳性，阴性3例；“金标准”诊断35例阴性中HpSA检测有33例阴性，阳性2例。HpSA检测的敏感性为93.0%(40/43)、特异性为94.3%(33/35)、诊断的准确性为93.6%(73/78)，阳性预测值为95.2%(40/42)和阴性预测值为91.7%(33/36)。结论：HpSA检测是一种简便、易行、准确的诊断儿童和老年患者Hp感染的非侵入性方法。

    关键词： 幽门螺杆菌； 粪便； 诊断； 酶联免疫法

    Diagnostic Value of Helicobacter Pylori Stool Antigen Assay in Childen and old People

    LIN Man-hua, YAO Yun-hong, et al

    (Institute of Laboratory Medicine of Guangdong Medical College, Guangdong Zhanjiang 524023, China)

    Abstract:Objective：To study applicated value of helicobacter pylori stool antigen(HpSA) assay in childen and old people. Methods：Stool specimens were measured by enzyme-linked immunosorbent assay(ELISA) in 30 cases childen and 48 cases old people who received upper gastrointestinal endoscopic examination for gastrointestinal symptoms. The evaluation of helicobacter pylori status was based on three different tests: rapid urease test, bacterial culture and histologic Warthin-Starry(WS) staining smear. Helicobacter pylori status was defined as positive when rapid urease test, the bacterial culture and/or histologic Warthin-Starry staining smear were positive. Results：In all patients ......