Protective effect of tetramethlpyrazine on coronary artery endothelium and cardiac muscle after LAD ligation

    HE Weiª²Lai, CHEN Ruª²Kun, ZHOU Ruª²Yuan

    1Department of Cardioª²thoracic Surgery, Second Hospital, Medical School, Zhejiang University, Hangzhou 310009, China, 2Department of Cardiovascular Surgery, First Hospital, Anhui Medical University, Hefei 230030, China

    ¡¾Abstract¡¿ AIM: To explore the protective effect of tetramethlpyrazine on the endothelium and cardiac muscle after acute infarction induced in dogs by ligating the left anterior descending coronary artery (LAD). METHODS: Fortyª²five aged healthy crossª²bred dogs (15¡À7) kg were divided into 3 groups: tetramethlpyrazine (TMP) group, verapamil (Ver) group and control group. The operation was performed to ligate the near part of the LAD through left minimal thoracic incision to block 75% of the blood flow respectively. The expression changes of endothelium derived factors NO, ETª²1, sPª²selectin and CTnT before ligation and at different time points after ligation were detected and Pª²selectin gene of cardiac muscle was determined by Western blotting. RESULTS: The difference was significant among the 3 groups at different time points after ligating LAD (P<0.01). The expression of Pª²selectin of cardiac muscle was highly upª²regulated after ligation in Ver group and control group. CONCLUSION: Tetramethlpyrazine can effectively restrain the injury of ischemia and reperfusion on endothelium of coronary artery and myocardial cells after ligating LAD and mitigate the stenosis of coronary artery and myocardial infarction, thus preventing the restenosis of coronary artery after coronary artery bypass graft. ......