Different expression and clinical application of CEAmRNAª²CEAp system in benign and malignant ascites

    ZHANG Jianª²Hua, ZHONG Huaiª²Yin, XUE Chengª²Yan

    Third Department of Infernal Medicine, Affiliated Hospital, Chengde Medical College, Chengde 067000, China

    ¡¾Abstract¡¿ AIM: To understand the different expression of carcinoembryonic antigen mRNA (CEAmRNA)ª²carcinoembryonic antigen protein (CEAp) system in benign and malignant ascites and to assess the value of CEAmRNAª²CEAp for the diagnosis and differential diagnosis of benign and malignant ascites. METHODS: Ninetyª²three patients were examined, among whom 60 cases were with malignant ascites and 33 cases with benign ascites. The ascites were collected and used for CEAmRNA and CEAp detection. CEAmRNA was detected by reverse transcriptaseª²nested primersª²polymerase chain reaction (RTª²NPª²PCR) and CEAp was detected by magnetic antibody immunoenzymometric assay (MAIA). RESULTS: The positive rates of CEAmRNA and CEAp were 49 (81.7%) and 34 (56.7%) in the malignant ascites group and 1 (3.0%) and 3 (9.1%) in the benign ascites group.The positive rates of both CEAmRNA and CEAp in the ascites of patients with malignant ascites were significantly higher than those of benign patients (P<0.01). In the diagnosis and differential diagnosis of malignant ascites, the specificity, sensitivity, the positive likelihood ratio, the negative likelihood ratio, the positive predictive value, the negative predictive value and the accuracy of CEAmRNA and of CEAp were respectively [0.970 and 0.909], [0.817 and 0.567], [27.233 and 6.231], [0.189 and 0.476], [0.980 and 0.919], [0.744 and 0.544], [0.871 and 0.699]. CONCLUSION: Both CEAmRNA and CEAp are useful indexes for the diagnosis and differential diagnosis of malignant ascites. The diagnostic efficiency of CEAmRNA is higher than that of CEAp. ......