Immunosuppressive effect of free hemoglobin on lymphocyte transformation function

    LUO Qun, ZHANG Xianª²Qing, LIU Jingª²Han

    1Department of Blood Transfusion, PLA General Hospital ,Beijing 100853, 2Department of Blood Transfusion , Xijing Hospital, Fourth Military Medical University, Xi¬ðan 710033, China

    ¡¾Abstract¡¿ AIM: To study the immunosuppressive effect of free hemoglobin on lymphocyte transformation function. METHODS: Using twoª²way mixed lymphocyte reaction (MLR), cell culture and incorporation of 3Hª²thymidine (3Hª²TdR), we added free hemoglobin (FHb) at different concentrations to the reaction system and raised the concentration of antigen presentation cells (APC) with normal APC concentration MLR as negative control and cyclosporinª²A (CsA) as positive control. The ratio of the above factors in suppressing MLR was calculated. RESULTS: The suppressive effect of FHb increased with its concentration. When the concentration of APC in the system was twice that of the normal, the suppressive effect of free hemoglobin became weaker. CONCLUSION: Free hemoglobin may suppress MLR by disturbing the APC function. We can use this method to study the immunomodulation of large volume blood transfusion. ......